Biodistribution of Gadolinium-DOTA-PAMAM Dendrimer Generation 3.0-Trastuzumab in Mice Organs

Deasy Biantong¹ and Hari Soekersi^1,

¹Department of Radiology, Dr. Hasan Sadikin General Hospital, Universitas Padjadjaran, Bandung, Indonesia

Cite this paper

Deasy Biantong and Hari Soekersi. Biodistribution of Gadolinium-DOTA-PAMAM Dendrimer Generation 3.0-Trastuzumab in Mice Organs. American Journal of Clinical Medicine Research. 2017; 5(3):36-38. doi: 10.12691/AJCMR-5-3-3

Abstract

To know the biodistribution of compounds Gadolinium-DOTA-PAMAM dendrimer generation 3.0-Trastuzumab as a necessary ingredient MRI contrast agents in the organs of mice. Biodistribution test using the compound with radioactive marker 125I is injected intravenously into the blood vessel and distributed to the organs of mice were detected by gamma ray count tool. This research method is a descriptive study. Sampling applied with purposive random sampling technique. Research conducted in the laboratory animal center of Radioisotopes and Radiopharmaceuticals Technology National Nuclear Energy Agency (BATAN PTRR), Serpong, Indonesia. Analysis univariable illustrates the biodistribution of compounds Gadolinium-DOTA-PAMAM dendrimer G 3.0-trastuzumab in the organs of healthy mice regarding percentage per gram organ, bivariable analysis to assess differences in the biodistribution of Gadolinium-DOTA-PAMAM dendrimer G 3.0-Trastuzumab in the organs of mice. Biodistribution differences of Gadolinium-DOTA-PAMAM dendrimer G 3.0-trastuzumab injected intravenously in the organs of mice through percentage per gram organs. Percentage per gram organ compounds injected are highest in the blood, the peak accumulation is at 3 hours and declined in 72 hours, the smallest is in the brain. The liver is the largest organ of elimination for the compound (Gd-DOTA)n-PAMAM dendrimer G3.0-Trastuzumab-125I, peak accumulation is at 3 hours and decreased at 72 hours, then the kidney with the highest accumulated peak at 1 hour and declined to 72 hours.

Keywords

biodistribution, Gadolinium-DOTA-PAMAM dendrimer G 3.0-Trastuzumab, Magnetic resonance imaging, mice

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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